Assessment of Bacteriocin-Antibiotic Synergy for the Inhibition and Disruption of Biofilms of Listeria monocytogenes and Vancomycin-Resistant Enterococcus

In this study, we have evaluated the effects of previously characterized bacteriocins produced by E. faecium strains ST651ea, ST7119ea, and ST7319ea, against biofilm formation biofilms formed L. monocytogenes ATCC15313 vancomycin-resistant VRE19. The on ATCC151313 were crystal violet assay further confirmed quantifying viable cells cell metabolic activities through flow cytometry TTC assay, respectively, indicating that bacteriocin required to completely eradicate are at least 1600 AU mL−1, 3200 6400 respectively for each evaluated. Furthermore, ST651ea ST7119ea require mL−1 viability within VRE19, while ST7319ea requires 12800 obtain same observations. Assessment synergistic between selected conventional antibiotics (ciprofloxacin vancomycin) with these was carried out evaluate their pre-formed both test microorganisms. Results showed higher concentrations needed in contrast abilities strains. ciprofloxacin vancomycin more evident VRE19 rather than ATCC15313. These observations can be explored possible applications combinations as a treatment clinically relevant pathogens.